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Gel Electrophersis
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2. Steps for Southern Blotting
• Digest the DNA with an appropriate restriction enzyme.
• Run the digest on an agarose gel.
• Denature the DNA, which would separate double-stranded DNA into single-stranded DNA.
• Transfer the denatured DNA to the membrane.
• Probe the membrane.
• Visualize your radioactively labeled target sequence. If you used a radiolabeled 32P probe, then you would visualize by autoradiograph.
3. a) She placed the nucleotide as she did because the EcoR I cuts...
Approximate word count: 590
Approximate pages: 3 (250 words per
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